ChromasPro is for assembly of sequence reads into contigs, with a graphical contig editor which displays aligned chromatograms. It also peforms general sequence analysis such as restriction enzyme mapping, open-reading-frame searches, and BLAST submission.
About this app
ChromasPro is suitable for DNA sequence assembly projects up to a few megabases, and basic sequence editing and analysis. It is able to assemble data from Sanger sequencers such as ABI, and 454 and Illumina next-generation sequencers, with up to 1,000,000 sequences if 8 Gb RAM is available. ChromasPro has the following features:
- Open sequences in Applied Biosystems .ab1, Staden Chromatogram (SCF and ZTR), 454 SFF, FASTA, FASTQ, EMBL, GenBank, SwissProt, GenPept, GCG RSF and plain text formats.
- Save sequences in .ab1, .scf, .fasta and .fastq formats.
- Assemble overlapping sequences using a graphical interface, generate a consensus and highlight ambiguities for editing.
- Use quality data to automatically remove low quality sequence for improved sequence assembly.
- Use a reference sequence as a scaffold for assembly.
- Generate restriction site and fragment maps, and list cutters, non-cutters and fragments.
- Map open reading frames, aided by a G+C frame plot, and translate ORFs with one click.
- Print chromatograms, restriction site and fragment maps, and open reading frame maps.
- Perform nucleotide and protein BLAST searches through the NCBI web site.
- Perform multiple alignments by interfacing with ClustalW.
- Reverse & complement sequences and chromatograms.
- Search for sequences by exact matching or optimal alignment.
- Display translations when editing nucleotide sequences.
- Perform reverse translations and plot nucleotide degeneracy.
- Plot hydrophilicity and antigenicity of proteins.
- Copy an image of a chromatogram section for pasting into documents or presentations.
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